Evidence map›Paper›PMID 42681277›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Purification and Analysis of Signaling Molecules in Plant-Arbuscular Mycorrhizal Fungal Communication.

Carlos Rial, Alexandra G Durán, José M G Molinillo, Juan A López-Ráez, Francisco A Macías, Rosa M Varela

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Carlos RialAllelopathy Group, Department of Organic Chemistry, Institute of Biomolecules (INBIO), School of Sciences, University of Cadiz, Puerto Real, Cadiz, Spain. carlos.rial@gm.uca.es.
Alexandra G DuránAllelopathy Group, Department of Organic Chemistry, Institute of Biomolecules (INBIO), School of Sciences, University of Cadiz, Puerto Real, Cadiz, Spain.
José M G MolinilloAllelopathy Group, Department of Organic Chemistry, Institute of Biomolecules (INBIO), School of Sciences, University of Cadiz, Puerto Real, Cadiz, Spain.
Juan A López-RáezDepartment of Soil and Plant Microbiology, Estación Experimental del Zaidín (EEZ-CSIC), Granada, Spain.
Francisco A MacíasAllelopathy Group, Department of Organic Chemistry, Institute of Biomolecules (INBIO), School of Sciences, University of Cadiz, Puerto Real, Cadiz, Spain.
Rosa M VarelaAllelopathy Group, Department of Organic Chemistry, Institute of Biomolecules (INBIO), School of Sciences, University of Cadiz, Puerto Real, Cadiz, Spain.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The arbuscular mycorrhizal (AM) symbiosis is the most widespread mutualistic association between plants and fungi. The most widely recognized benefit of AM symbiosis for the host plant is a significant enhancement in the acquisition of mineral nutrients from the soil, particularly phosphorus. In addition to improved nutrition, AM symbiosis confers enhanced tolerance to a wide range of environmental stresses. Strigolactones (SLs) are a family of natural products produced by plants as shoot branching factors and are responsible for the induction of hyphal branching in arbuscular mycorrhizal fungi (AMF). On the other hand, flavonoids also play crucial roles in various signaling processes, such as legume-rhizobia symbiosis or in the AM symbiosis. Being able to accurately analyze strigolactones and flavonoids is essential for unraveling the basic mechanisms of AM symbiosis. However, the low amount of them produced by plants and their rapid degradability make it crucial to develop fast analytical methods with very low limits of quantification to study the interaction and communication between plants and AMF. Herein, a protocol is described for the development of a LC-MS/MS method for the quantification of SLs and flavonoids, using GR24 and chrysin as internal standards, respectively, in roots, exudates, and extracts.

Indexed as

LactonesMycorrhizaePlantsChromatography, LiquidFlavonoidsLiquid Chromatography-Mass SpectrometryPlant RootsSignal TransductionSymbiosisTandem Mass SpectrometryFlavonoidsLactonesArbuscular mycorrhizal fungiCarotenoidsFlavonoidsLC–MS/MSQuantification methodStrigolactones

Identifiers

PMID42681277

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.