ArticleMethods in molecular biology (Clifton, N.J.)2026
A Whole-Plant Culture Method to Study Structural and Functional Traits of Extraradical Mycelium.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An in vivo whole-plant bi-dimensional experimental system has been devised and tested with different host plants to obtain extraradical mycelium (ERM) produced by different isolates of arbuscular mycorrhizal fungi (AMF). In this system, a plantlet is inoculated with AMF to establish mycorrhizal symbiosis and, after colonization, newly formed extraradical hyphae and spores are removed. Then, the mycorrhizal root system is wrapped in a nylon net and placed between two membranes in a Petri dish, allowing ERM to grow on the membrane surfaces. Such extraradical hyphae may be used for in situ morphometric analyses or harvested for molecular and biochemical assays; in the latter case, when reassembled, the whole-plant system can allow new mycelial harvests up to a maximum plant lifespan of 6 months. In this experimental system, which was tested with diverse host plant species and lines, values of explored membrane surface areas and densities of ERM showed large variations, and their length ranged from 9.7 ± 2.0 to 120.1 ± 14.1 m per plant, depending on host and AMF identity. Across the different plant-AMF combinations tested, the whole-plant system produced 2.0 ± 0.6 to 8.3 ± 3.3 mg of ERM fresh biomass per plant per harvest. This experimental system can be used for a wide range of AMF and host plant species, either establishing arbuscular mycorrhizas or other mycorrhizal interactions. ERM produced and collected in the whole-plant system is suitable for morphological, physiological, and molecular analyses, facilitating studies on the different aspects of mycorrhizal symbiotic interactions.
Indexed as
Identifiers
42681270What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.