ArticleMethods in molecular biology (Clifton, N.J.)2026
Methods for Single-Spore Cultures of Arbuscular Mycorrhizal Fungi Using a Superabsorbent Polymer-Based Autotrophic System.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Single-spore cultures of arbuscular mycorrhizal fungi (AMF) are essential for taxonomy, genomics, experimental ecology, and the production of well-characterized inoculum. Classical pot cultures in opaque substrates make monitoring the establishment of symbiosis and verifying culture purity difficult, whereas in vitro cultures on Ri T-DNA-transformed roots require sterile conditions and support only a limited subset of AMF species. Transparent "soils" based on superabsorbent polymers (SAPs) offer a simple alternative that combines the advantages of in vivo and in vitro systems. Here, we present the superabsorbent polymer-based autotrophic system (SAP-AS), a simple and inexpensive culture system. The SAP-AS allows for easy single-spore inoculation, monitoring, and maintenance.
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