Evidence map›Paper›PMID 42681256›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Cloning and Characterizing Multidrug Efflux Pumps.

Martijn Zwama, Kunihiko Nishino

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Martijn ZwamaSANKEN (The Institute of Scientific and Industrial Research), The University of Osaka, Ibaraki, Osaka, Japan. m.zwama@sanken.osaka-u.ac.jp.ORCID https://orcid.org/0000-0001-9081-382X
Kunihiko NishinoSANKEN (The Institute of Scientific and Industrial Research), The University of Osaka, Ibaraki, Osaka, Japan.ORCID https://orcid.org/0000-0003-3349-1769

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This protocol describes how to clone and investigate multidrug efflux pumps from plasmids to be expressed in E. coli and P. aeruginosa. We want to provide a quick overview for those starting with RND-type efflux pump research or facing cloning issues by sharing an updated method we have recently been using to clone multiple functional pumps and complex components quickly and efficiently.

Indexed as

Bacterial ProteinsCloning, MolecularDrug Resistance, Multiple, BacterialMembrane Transport ProteinsAnti-Bacterial AgentsEscherichia coliPlasmidsPseudomonas aeruginosaAnti-Bacterial AgentsBacterial ProteinsMembrane Transport ProteinsAntibiotic resistanceAntibioticsDrug effluxFunctional analysisMolecular cloningMultidrug exporterMultidrug resistanceResistance–nodulation–cell divisionRND superfamily

Identifiers

PMID42681256

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.