Evidence map›Paper›PMID 42681254›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Mapping Genes Controlling Bacterial Cell Permeability Using TraDISort.

Liping Li, Evan Gibbs, Francesca Short, Claire Maher, Amy K Cain, Ian T Paulsen, Karl A Hassan

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Liping LiARC Centre of Excellence in Synthetic Biology, Macquarie University, Sydney, NSW, Australia.
Evan GibbsARC Centre of Excellence in Synthetic Biology, Macquarie University, Sydney, NSW, Australia.
Francesca ShortSchool of Natural Sciences, Macquarie University, Sydney, NSW, Australia.
Claire MaherARC Centre of Excellence in Synthetic Biology, Macquarie University, Sydney, NSW, Australia.
Amy K CainARC Centre of Excellence in Synthetic Biology, Macquarie University, Sydney, NSW, Australia.
Ian T PaulsenARC Centre of Excellence in Synthetic Biology, Macquarie University, Sydney, NSW, Australia.
Karl A HassanARC Centre of Excellence in Synthetic Biology, Macquarie University, Sydney, NSW, Australia. karl.hassan@newcastle.edu.au.ORCID https://orcid.org/0000-0003-2031-9679

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bacterial cell envelopes have evolved to control the passage of small molecules into and out of the cell. Understanding these barriers and the pathways that small molecules can take as they cross the barrier could help to improve outcomes in broad areas of applied microbiology, e.g., to improve antibiotic uptake for infectious disease treatment, to control pollutant uptake in bioremediation, and to enhance bioproduct export in industrial fermentation. We developed an innovative high-throughput screening method that can be used to discover novel bacterial genes that affect intracellular accumulation of small molecules, such as efflux or uptake transporter genes, or genes controlling cell membrane composition. This method is a combination of Transposon Directed Insertion site Sequencing and fluorescence-activated cell sorting through flow cytometry (TraDISort). TraDISort has broad applications beyond studies of cell permeability, but in this chapter, we provide a basic protocol to study the penetration of fluorescent compounds.

Indexed as

BacteriaCell Membrane PermeabilityFlow CytometryGenes, BacterialAnti-Bacterial AgentsDNA Transposable ElementsMutagenesis, InsertionalAnti-Bacterial AgentsDNA Transposable ElementsAntibioticAntimicrobial resistanceCell permeabilityEfflux pumpsFluorescence-activated cell sorting (FACS)Metabolite accumulationTraDISTransposon mutagenesis

Identifiers

PMID42681254

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.