Crystallographic Studies of RND- and ABC-Type Tripartite Multidrug Transporters.

Ui Okada, Satoshi Murakami

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

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2 · The registry

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

2 authors.

Ui OkadaDepartment of Life Science and Technology, Institute of Science Tokyo, Yokohama, Japan. uiokada@life.isct.ac.jp.

Satoshi MurakamiDepartment of Life Science and Technology, Institute of Science Tokyo, Yokohama, Japan. murakami@life.isct.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Three-dimensional structural information is crucial for the precise and detailed investigation of protein functions and their molecular mechanisms. X-ray crystallography is an effective method for determining protein structures. Here, we describe our standardized protocols, including overexpression, purification, crystallization, and handling of crystals for the structural analysis of multidrug transporters. We have determined several crystal structures of resistance-nodulation-division (RND) and ATP-binding cassette (ABC)-type tripartite multidrug transporters using these methods. These results facilitate the understanding of the multidrug resistance molecular mechanisms, which are mediated by tripartite multidrug efflux complexes.

Indexed as

ATP-Binding Cassette TransportersBacterial ProteinsMembrane Transport ProteinsCrystallizationCrystallography, X-RayProtein ConformationATP-Binding Cassette TransportersBacterial ProteinsMembrane Transport ProteinsABC transportersMembrane proteinMultidrug resistanceRND transportersTripartite multidrug efflux complexX-ray crystallography

Identifiers

PMID42681243

DOI10.1016/j.pep.2005.01.016

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.