ArticleMethods in molecular biology (Clifton, N.J.)2026
Producing Gynogenetic Embryos in Xenopus tropicalis.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Gynogenesis is a form of parthenogenesis in which sperm is required to activate the egg but contributes no genetic material to the embryo. In Xenopus, gynogenetic embryos can be created by using UV-irradiated sperm in in vitro fertilization and then preventing the formation of the second polar body, so the embryo retains two maternal chromosomes. The method is very simple, relies on standard laboratory equipment, and can be used to create homozygous embryos from heterozygous female carriers without the need to raise an F1 generation for in-crosses.
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