Evidence map›Paper›PMID 42681232›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Automated Behavioral Tracking and Analysis of Xenopus Tadpoles.

Sulagna Banerjee, Stefanie Neupert, Paul Szyszka, Caroline W Beck

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Sulagna BanerjeeDepartment of Zoology, University of Otago, Dunedin, 9016, New Zealand. sulagna.banerjee@otago.ac.nz.
Stefanie NeupertDepartment of Zoology, University of Otago, Dunedin, 9016, New Zealand.
Paul SzyszkaDepartment of Zoology, University of Otago, Dunedin, 9016, New Zealand.
Caroline W BeckDepartment of Zoology, University of Otago, Dunedin, 9016, New Zealand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Automated behavioral tracking provides a quantitative and reproducible way to measure locomotor activity in Xenopus tadpoles. We describe a method for simultaneously recording 24-free swimming tadpoles in 24-well plates under back-light illumination using a Raspberry Pi-based computer and camera. Locomotion is analyzed using TopScan tracking software, which identifies the center mass for each tadpole within its arena. The protocol quantifies locomotor parameters such as swim distance, velocity, and activity patterns. We outline steps required to assemble and calibrate the imaging hardware, configure the recording script, and process video data for downstream behavioral analysis. Together, this workflow provides a reliable foundation for studying locomotor behavior and detecting alterations arising from genetic or pharmacological manipulations.

Indexed as

Behavior, AnimalLarvaLocomotionXenopus laevisAnimalsImage Processing, Computer-AssistedMotion CaptureSoftwareSwimmingVideo RecordingAutomated trackingBehavioral analysisLocomotor activityTadpole behavior

Identifiers

PMID42681232

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.