Evidence map›Paper›PMID 42681228›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Fluorescent In Situ mRNA Hybridization (FISH) Using Hybridization Chain Reaction (HCR) in Xenopus Cryosections.

David Vijatovic, Stavros Papadopoulos, Marco Dalla Vecchia, Lora B Sweeney

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

David Vijatovic *Institute of Science and Technology Austria (ISTA), Am Campus 1, Klosterneuburg, 3400, Austria.
Stavros Papadopoulos *Institute of Science and Technology Austria (ISTA), Am Campus 1, Klosterneuburg, 3400, Austria.
Marco Dalla VecchiaInstitute of Science and Technology Austria (ISTA), Am Campus 1, Klosterneuburg, 3400, Austria.
Lora B SweeneyInstitute of Science and Technology Austria (ISTA), Am Campus 1, Klosterneuburg, 3400, Austria. lora.sweeney@ista.ac.at.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hybridization Chain Reaction (HCR) enables highly sensitive and multiplexed detection of mRNA with subcellular spatial resolution. It employs fluorophore-tagged DNA hairpins that self-assemble on target-bound probe pairs, amplifying the signal without enzymatic reactions. Here, we describe a protocol for performing HCR fluorescence in situ hybridization (FISH) on Xenopus tissue sections. We also outline a semi-automated image analysis pipeline that enables per-cell quantification of probe expression and co-localization. This method provides a robust and quantitative approach for visualizing gene expression patterns in situ.

Indexed as

In Situ Hybridization, FluorescenceRNA, MessengerXenopusAnimalsCryoultramicrotomyFluorescent DyesFluorescent DyesRNA, MessengerFluorescence microscopyHybridization chain reactionIn situ hybridizationmRNA FISH

Identifiers

PMID42681228

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.