ArticleMethods in molecular biology (Clifton, N.J.)2026
Whole Mount In Situ Hybridization in Xenopus.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Authors and funding
2 authors.
Funding
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Abstract
Whole mount in situ hybridization is a method of viewing gene expression in intact tissue, developing embryos, and tadpoles. It uses labeled, in vitro transcribed RNA probes to specifically bind to mRNA through complementary base pairing. Unlike protein detection, making an antisense RNA probe requires only knowledge of the corresponding coding sequence. It is therefore accessible for any gene, and has contributed hugely to our understanding of how genes shape development. This protocol highlights the key considerations balancing fixation and permeabilization to preserve tissue while allowing the probe to enter cells, and to optimise signal-to-noise for the colorimetric reaction.
Indexed as
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42681227What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.