ArticleMethods in molecular biology (Clifton, N.J.)2026
Cryogenic Preservation of Xenopus Sperm.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
2 authors.
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Abstract
Cryopreservation of Xenopus sperm offers several advantages: it reduces the number of live animals needed and maintained for in vitro fertilization, enables efficient archiving of genetically altered lines, and facilitates easy transfer between laboratories, lowering animal suffering and overall costs. Sperm freezing and in vitro fertilization using frozen sperm are simple procedures that require only standard laboratory equipment. Testes are macerated in Leibovitz L-15 medium, combined with a cryoprotectant made from egg yolk and sucrose and slowly frozen overnight in a polystyrene box at -80°C.
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Registered trials
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