Site-Specific Dual-Color Labeling of Long RNAs.

Niko S W Lindlar Né Jonasson, Meng Zhao, Richard Börner, Roland K O Sigel, Eva Freisinger

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Niko S W Lindlar Né JonassonDepartment of Chemistry, University of Zurich, Zurich, Switzerland.

Meng ZhaoHangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou, Zhejiang, China.

Richard BörnerLaserinstitut Hochschule Mittweida (LHM), Mittweida University of Applied Sciences, Mittweida, Germany.

Roland K O SigelDepartment of Chemistry, University of Zurich, Zurich, Switzerland. roland.sigel@chem.uzh.ch.

Eva FreisingerDepartment of Chemistry, University of Zurich, Zurich, Switzerland. freisinger@chem.uzh.ch.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Labeling of large RNAs with reporting entities, for example, fluorophores, has significant impact on RNA studies in vitro and in vivo. Here we describe a minimally invasive RNA labeling method featuring nucleotide- and position-selectivity, which solves the long-standing challenge of how to achieve accurate site-specific labeling of large RNAs with a least possible influence on folding and/or function. We use a custom-designed reactive DNA strand to hybridize to the RNA and transfer the alkyne group onto the targeted adenine or cytosine. Simultaneously, the 3'-terminus of RNA is converted to a di-aldehyde moiety under the experimental condition applied. The incorporated functionalities at the internal and the 3'-terminal sites can then be conjugated with reporting entities via bioorthogonal chemistry. This method is particularly valuable for, but not limited to, single-molecule fluorescence applications. We demonstrate the method on an RNA construct of 275 nucleotides, the btuB riboswitch of Escherichia coli.

Indexed as

Staining and LabelingEscherichia coliFluorescence Resonance Energy TransferFluorescent DyesNucleic Acid HybridizationRiboswitchRNAFluorescent DyesRiboswitchRNALong RNAsOrthogonal chemistryRiboswitchSingle-molecule fluorescence resonance energy transfer (smFRET)Site-specific labeling

Identifiers

PMID42681207

DOI10.1007/978-1-0716-0231-7_16

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.