Delivering Molecular Beacons via an Electroporation-Based Approach Enables Live-Cell Imaging of Single RNA Transcripts and Genomic Loci.

Shiqi Mao, Yachen Ying, Xiaotian Wu, Antony K Chen

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 authors.

Shiqi MaoDepartment of Biomedical Engineering, College of Future Technology, Peking University, Beijing, China.

Yachen YingDepartment of Biomedical Engineering, College of Future Technology, Peking University, Beijing, China.

Xiaotian WuDepartment of Biomedical Engineering, College of Future Technology, Peking University, Beijing, China.

Antony K ChenDepartment of Biomedical Engineering, College of Future Technology, Peking University, Beijing, China. chenak@pku.edu.cn.

Funding

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6 · The paper itself

Abstract

Molecular beacons (MBs) are synthetic oligonucleotide probes that are designed to fluoresce upon hybridization to complementary nucleic acid targets. In contrast to genetically encoded probes that can be readily introduced into cells via standard transfection procedures, using MBs to obtain reliable intracellular measurements entails a reliable delivery method that maximizes MB entry while minimizing cell damage. One promising approach is microporation, a microliter volume electroporation-based method that exhibits reduced harmful events as compared with traditional electroporation methods. In this chapter, we describe in detail microporation steps for MB delivery that we have utilized over the past several years, followed by examples demonstrating successful MB-based imaging of specific RNA transcripts and genomic loci at the single-molecule level.

Indexed as

ElectroporationGenetic LociOligonucleotide ProbesRNA, MessengerSingle Molecule ImagingAnimalsHumansNucleic Acid HybridizationTransfectionOligonucleotide ProbesRNA, MessengerMicroporationMolecular beaconsSingle-moleculeTandem repeats

Identifiers

PMID42681206

DOI10.1007/978-1-4939-7213-5_15

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.