Evidence map›Paper›PMID 42681188›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Solid Phase Chemical Synthesis of RNA.

Joshua O'Grady, Zidi Lyu, Masad J Damha, David Sabatino

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Joshua O'GradyDepartment of Chemistry, Ottawa, ON, Canada.
Zidi LyuDepartment of Chemistry, McGill University, Montreal, QC, Canada.
Masad J DamhaDepartment of Chemistry, McGill University, Montreal, QC, Canada.
David SabatinoDepartment of Chemistry, Ottawa, ON, Canada. david.sabatino@carleton.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Solid phase oligonucleotide synthesis enables the generation of various biologically relevant RNAs. These include RNA sequences 20-80 nucleotides in length, such as microRNA (miRNA), small interfering RNA (siRNA), self-amplifying RNA (saRNA), and RNA aptamers. Modern advances in RNA chemical synthesis have also produced longer RNA sequences (≥100 nucleotides), such as guide RNA (gRNA), messenger RNA (mRNA), riboswitches, and ribozymes. Further, the introduction of orthogonal protecting and reactive groups allows for the chemical synthesis of branch RNA as mimics of intronic RNA lariats. Here, we describe a solid phase chemical synthesis protocol based on t-butydimethylsilyl (TBDMS) phosphoramidite chemistry to produce native or branched RNA suitable for structural, functional, or biological studies.

Indexed as

RNASolid-Phase Synthesis TechniquesOligonucleotidesOrganophosphorus CompoundsOligonucleotidesOrganophosphorus CompoundsphosphoramiditeRNABranch and lariat RNAPhosphoramidite chemistryRNASolid phase oligonucleotide synthesisTherapeutic oligonucleotides

Identifiers

PMID42681188

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.