Targeted Transition Mutations in Phage-Assisted Evolution.

Thandava Vanapilli Nursimulu, Maryam Ali, Jumi A Shin

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

3 authors.

Thandava Vanapilli NursimuluDepartment of Chemistry, University of Toronto, Mississauga, ON, Canada.

Maryam AliDepartment of Chemistry, University of Toronto, Mississauga, ON, Canada.

Jumi A ShinDepartment of Chemistry, University of Toronto, Mississauga, ON, Canada. jumi.shin@utoronto.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Phage-assisted evolution is a powerful strategy for directed evolution in which the protein undergoes continuous mutagenesis under selective pressure to rapidly evolve protein variants. However, conventional approaches often introduce mutations across the entire target plasmid, not just the gene-of-interest (GOI), leading to unwanted genomic changes that enable the evasion of selective pressure. To overcome this limitation, we integrated the MutaT7 system, which uses two base deaminases, into phage-assisted evolution to direct all four transition mutations specifically to the GOI. By using these deaminases, our system promotes the accumulation of all transition mutations, providing greater diversification of the GOI. This targeted yet comprehensive mutation strategy accelerates and enhances the evolutionary outcome of proteins to improve their stability, binding affinity, and specificity.

Indexed as

Bacteriophage M13BacteriophagesDirected Molecular EvolutionMutationBacteriophage T7MutagenesisPlasmidsPromoter Regions, GeneticDeaminasesDirected evolutionM13 bacteriophagePhage-assisted evolutionSelectionT7 promoterT7 RNAPT7 terminatorTargeted mutagenesis

Identifiers

PMID42681185

DOI10.1073/pnas.86.7.2172

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.