A Yeast Two-Hybrid Assay to Develop Optogenetic Tools for Controlling Protein-Protein Interactions.

Ryan M Woloschuk, G Andrew Woolley

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Ryan M WoloschukJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.

G Andrew WoolleyDepartment of Chemistry, University of Toronto, Toronto, ON, Canada. andrew.woolley@utoronto.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Yeast two hybrid (Y2H) assays serve as powerful platforms for the detection and engineering of protein-protein interactions. Here, we describe a strategy for using Y2H-based screening to assess and develop optogenetic tools based on light-controlled protein-protein interactions.

Indexed as

OptogeneticsProtein Interaction MappingTwo-Hybrid System TechniquesHigh-Throughput Screening AssaysProtein BindingProtein EngineeringSaccharomyces cerevisiaeHigh-throughput screeningLibrary screeningOptogeneticsPhotoswitchable proteinsProtein engineeringYeast two hybrid

Identifiers

PMID42681176

DOI10.1016/j.biotechadv.2021.107762

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.