Evidence map›Paper›PMID 42681156›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Atomic Force Microscopy Investigations of Collagen: From Single Molecules to Fibrils.

Janine Srocka, Alaa Al-Shaer, Nancy R Forde

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. On-glass Interference Confocal Reflectance Microscopy for Collagen Fibril Diameter Measurement.Proceedings of SPIE--the International Society for Optical Engineering · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Janine Srocka *Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada.
Alaa Al-Shaer *Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada.
Nancy R FordeDepartment of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada. nforde@sfu.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Collagen is a key component of the extracellular matrix, providing structural support and influencing cellular behavior through its physical properties. Understanding its mechanical properties is essential for elucidating its role in tissue integrity and pathology. Collagen forms complex hierarchical structures, ranging from individual proteins to higher-order fibrils, fibers, and networks, each contributing to the mechanical stability of tissues. In this methods chapter, we outline the use of atomic force microscopy (AFM) to investigate collagen's structural properties across multiple hierarchical levels, from single molecules to fibrils. We describe the preparation of collagen samples for AFM analysis of collagen in three forms: proteins, reconstituted fibrils, and tendon-derived fibrils from rat tails. Additionally, we describe a MATLAB-based image analysis method for quantitatively assessing mechanical properties of individual collagen proteins.

Indexed as

CollagenMicroscopy, Atomic ForceSingle Molecule ImagingAnimalsExtracellular MatrixImage Processing, Computer-AssistedRatsTendonsCollagenAtomic force microscopy (AFM)CollagenFibrilsImage analysisMechanicsPersistence lengthProtein structureSingle-molecule imaging

Identifiers

PMID42681156

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.