Evidence map›Paper›PMID 42681142›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Efficient Establishment of Interfamily Heterograft of Nicotiana benthamiana and Arabidopsis thaliana.

Kentaro Okada, Koji Okayasu, Michitaka Notaguchi

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Kentaro OkadaBioscience and Biotechnology Center, Nagoya University, Nagoya, Japan.
Koji OkayasuGraduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan.ORCID http://orcid.org/0000-0002-0189-5964
Michitaka NotaguchiBioscience and Biotechnology Center, Nagoya University, Nagoya, Japan. notaguchi.michitaka.4k@kyoto-u.ac.jp.ORCID http://orcid.org/0000-0003-0831-2108

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The grafting technique has been applied to study systemic signaling in plants, to investigate whether gene action is graft-transmissible and/or gene products such as RNAs and proteins are transported systemically. Here, we describe two protocols, stem grafting and micrografting, for an interfamily heterograft system between Nicotiana benthamiana scion plants and Arabidopsis stock plants for the identification of systemic mobile signals. Since these plants belong to evolutionally distant families and both genome databases are available, this system can be used to identify mobile substances transported from one plant to the other without doubt.

Indexed as

ArabidopsisNicotianaPhloemPlants, Genetically ModifiedPlant StemsArabidopsisGraftingHeterograftInterfamily graftingMobile signalsNicotianaPhloem transportWedge-graft

Identifiers

PMID42681142

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.