Evidence map›Paper›PMID 42681122›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Visual Detection of Inorganic Orthophosphate in Phloem Cells.

Wenyuan Ruan, Meina Guo, Qianqian Zhang, Keke Yi

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Wenyuan RuanState Key Laboratory of Efficient Production of Forest Resources/National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China. ruanwenyuan@caas.cn.
Meina GuoInstitute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China.
Qianqian ZhangState Key Laboratory of Efficient Production of Forest Resources/National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
Keke YiState Key Laboratory of Efficient Production of Forest Resources/National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The phloem plays a vital role in actively transporting soluble organic compounds-such as sugars, minerals, and proteins-from source to sink tissues, a process driven by turgor pressure and adenosine triphosphate (ATP)-dependent energy. Since ATP levels in phloem cells are closely linked to inorganic orthophosphate (Pi) availability, precise and efficient detection of Pi in these cells is essential for understanding phloem function. To address this, we developed the inorganic orthophosphate staining assay (IOSA), a method for visually detecting Pi in phloem cells. As a demonstration, we have successfully applied IOSA to the quantification of Pi levels in rice stem phloem cells.

Indexed as

OryzaPhloemPhosphatesStaining and LabelingAdenosine TriphosphateAdenosine TriphosphatePhosphatesInorganic orthophosphate (Pi)IOSAPhloem

Identifiers

PMID42681122

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.