Evidence map›Paper›PMID 42681119›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Live-Cell Imaging of Fluorescently-Tagged Phloem Proteins with Confocal Microscopy.

Thibaud Cayla, Rozenn Le Hir, Sylvie Dinant

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Thibaud CaylaInstitute Jean-Pierre Bourgin for Plant Sciences, Université Paris-Saclay, AgroParisTech-INRAE, Versailles, France.
Rozenn Le HirInstitute Jean-Pierre Bourgin for Plant Sciences, Université Paris-Saclay, AgroParisTech-INRAE, Versailles, France.
Sylvie DinantInstitute Jean-Pierre Bourgin for Plant Sciences, Université Paris-Saclay, AgroParisTech-INRAE, Versailles, France. sylvie.dinant@inrae.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Confocal laser scanning microscopy can enable observation of phloem cells in living tissues. Here, we describe live imaging of phloem cells in the leaves and roots of Arabidopsis thaliana using fluorescently-tagged proteins, either expressed in the vasculature using phloem-specific promoters or constitutively expressed in reference marker lines. We provide instructions for identifying the majority of phloem cell types and a step-by-step protocol for the precise cellular and subcellular localization of phloem proteins.

Indexed as

ArabidopsisArabidopsis ProteinsPhloemGreen Fluorescent ProteinsMicroscopy, ConfocalPlant LeavesPlant RootsArabidopsis ProteinsGreen Fluorescent ProteinsFluorescent markersGreen fluorescent proteinsLive-cell imagingMicroscopyMobile symplasmic probes

Identifiers

PMID42681119

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.