Evidence map›Paper›PMID 42681118›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Identification of Phloem Cell Transcriptome via Single-Cell RNA-Sequencing.

Sol-Bi Kim, Efthymia Symeonidi, Tin Yau Pang, Hongshi Jin, Ji-Yun Kim

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Sol-Bi KimDepartment of Biological Sciences, Sungkyunkwan University, Suwon, Republic of Korea.
Efthymia SymeonidiSchool of Biological Sciences, University of Utah, Salt Lake City, UT, USA.
Tin Yau PangGerman Diabetes Center (DDZ), Leibniz Institute for Diabetes Research, Düsseldorf, Germany.
Hongshi JinDepartment of Biological Sciences, Sungkyunkwan University, Suwon, Republic of Korea.
Ji-Yun KimDepartment of Biological Sciences, Sungkyunkwan University, Suwon, Republic of Korea. jiyun.kim@skku.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single-cell RNA sequencing (scRNA-seq) has emerged as a powerful tool for dissecting cellular heterogeneity in plants at a high resolution. In particular, scRNA-seq has been applied to unravel the complex cell identities and regulatory networks of distinct phloem cell types. Because phloem cells are small and under high osmotic pressure, isolating and maintaining viable protoplasts is a challenge for successful phloem scRNA-seq. Here, we present an optimized protocol for isolating vasculature-enriched protoplasts from mature Arabidopsis thaliana leaves. Following tissue harvesting, protoplasting is performed using a cocktail of cell wall-degrading enzymes suspended in an osmotically stabilized buffer. The resulting cell suspension is purified by removing debris through subsequent wash steps and validated using phloem-specific markers. Besides providing a reliable and reproducible method for isolating high-purity phloem-enriched protoplasts, we introduce the basic pipeline for downstream analysis.

Indexed as

ArabidopsisPhloemSequence Analysis, RNASingle-Cell AnalysisTranscriptomeGene Expression ProfilingPlant LeavesProtoplastsSingle-Cell Gene Expression AnalysisArabidopsisCell wall digestionPhloem transcriptomeProtoplast isolationSingle-cell RNA sequencing

Identifiers

PMID42681118

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.