Evidence map›Paper›PMID 42681093›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Nucleobase-Modified Triplex-Forming Peptide Nucleic Acids for Sequence-Specific Recognition of Double-Stranded RNA and DNA.

Sara Farshineh Saei, Brandon Tessier, James A MacKay, Eriks Rozners

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Sara Farshineh Saei *Department of Chemistry, The State University of New York, Binghamton, NY, USA.
Brandon Tessier *Department of Chemistry, The State University of New York, Binghamton, NY, USA.
James A MacKayDepartment of Chemistry and Biochemistry, Elizabethtown College, Elizabethtown, PA, USA.
Eriks RoznersDepartment of Chemistry, The State University of New York, Binghamton, NY, USA. erozners@binghamton.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Non-coding RNAs play important roles in the regulation of gene expression. Sequence-specific recognition of these regulatory RNAs is essential for both fundamental scientific research and the pharmaceutical industry. However, the complex helical structures formed by biologically relevant RNAs present a formidable challenge for molecular recognition. The present protocol describes nucleobase-modified triplex-forming peptide nucleic acids (PNAs) designed for sequence-specific recognition of double-stranded RNA and DNA under physiologically relevant conditions. Protocols for PNA synthesis and purification, as well as RNA binding studies using isothermal titration calorimetry and UV thermal melting, are described in detail. We also describe improved PNA bis-clamps with the ability to invade double-stranded DNA at physiological salt conditions.

Indexed as

DNAPeptide Nucleic AcidsRNA, Double-StrandedBase SequenceCalorimetryNucleic Acid ConformationNucleic Acid DenaturationDNAPeptide Nucleic AcidsRNA, Double-StrandedDouble-stranded RNAIsothermal titration calorimetry (ITC)Peptide nucleic acid (PNA)Triple helixUV thermal melting

Identifiers

PMID42681093

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.