Evidence map›Paper›PMID 42681092›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Near-Infrared In Vivo Whole Body Fluorescence Imaging of Peptide Nucleic Acid.

Ernest Wee Kiat Lim, Peter E Nielsen, Camilla Brolin

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Ernest Wee Kiat LimFaculty of Health and Medical Sciences, Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark.
Peter E NielsenFaculty of Health and Medical Sciences, Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark.
Camilla BrolinFaculty of Health and Medical Sciences, Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark. cbh@sund.ku.dk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Using near-infrared fluorophore Alexa Fluor-680 peptide nucleic acid (PNA) conjugates, the biodistribution of this potential antisense therapeutic agent can be analyzed in real time in live mice using in vivo imaging. By tracking the fluorescence intensity emitted from the mouse at different time points following administration, the systemic distribution and organ accumulation of PNA can be tracked. In addition, an estimation of the compound's body half-life can be obtained by the change in fluorescence intensity distribution over time. With this technique, the circulation of compounds can be monitored in real time while reducing the number of animals and amount of compounds required.

Indexed as

Optical ImagingPeptide Nucleic AcidsWhole Body ImagingAnimalsFluorescent DyesMiceSpectroscopy, Near-InfraredTissue DistributionFluorescent DyesPeptide Nucleic AcidsBiodistributionIVISTissue accumulationWhole body in vivo bioimaging

Identifiers

PMID42681092

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.