Evidence map›Paper›PMID 42681053›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Protein Extraction Methods for Ustilago maydis.

María Dolores Pejenaute Ochoa, José Ignacio Ibeas Corcelles, Ramón Ramos Barrales

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

María Dolores Pejenaute OchoaCentro Andaluz de Biología del Desarrollo (CABD), Universidad Pablo de Olavide-CSIC-Junta de Andalucía, Seville, Spain. mpejenauteochoa001@dundee.ac.uk.ORCID http://orcid.org/0000-0001-9289-3077
José Ignacio Ibeas CorcellesCentro Andaluz de Biología del Desarrollo (CABD), Universidad Pablo de Olavide-CSIC-Junta de Andalucía, Seville, Spain.ORCID http://orcid.org/0000-0002-2394-7075
Ramón Ramos BarralesCentro Andaluz de Biología del Desarrollo (CABD), Universidad Pablo de Olavide-CSIC-Junta de Andalucía, Seville, Spain. rrambar@upo.es.ORCID http://orcid.org/0000-0002-5256-3222

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Proteins perform essential structural, enzymatic, and regulatory functions, many of which depend on their subcellular localization. In plant-pathogen interactions, they are crucial in establishing virulence. The phytopathogenic fungus Ustilago maydis employs protein-based strategies, including effector secretion, cell wall remodeling, membrane-associated transport, and regulatory signaling mechanisms to infect the maize plant. To facilitate the study of these proteins, a set of optimized and reproducible protocols has been developed for isolating cytosolic, membrane-bound, secreted, and glycosylated protein fractions. These methods combine cryogenic disruption, specific lysis buffers, TCA-DOC precipitation, and affinity enrichment, providing a robust workflow for comprehensive protein analysis in fungal pathogenesis.

Indexed as

Fungal ProteinsUstilagoBasidiomycotaGlycosylationPlant DiseasesZea maysFungal ProteinsGlycosylationMembrane proteinsPlant-pathogenic fungiProtein extractionSecreted proteinsUstilago maydis.

Identifiers

PMID42681053

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.