Evidence map›Paper›PMID 42681052›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Immunodetection of Cell-Surface-Associated Proteins in Ustilago maydis and Infected Maize Leaves.

Chibbhi Bhaskar, Minh-Quang Chau, Lay-Sun Ma

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Chibbhi BhaskarInstitute of Plant and Microbial Biology, Academia Sinica, Taipei, 11529, Taiwan.
Minh-Quang ChauInstitute of Plant and Microbial Biology, Academia Sinica, Taipei, 11529, Taiwan.
Lay-Sun MaInstitute of Plant and Microbial Biology, Academia Sinica, Taipei, 11529, Taiwan. laysunma@as.edu.tw.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Immunolocalization relies on the sequential binding of a specific primary antibody to its target antigen, followed by detection with a fluorophore-conjugated secondary antibody to visualize the subcellular localization of proteins. This technique enables precise and high-resolution mapping of protein localization and distribution patterns after secretion. This chapter presents a detailed protocol for the immunolocalization of secreted proteins from Ustilago maydis, both in vitro and in infected maize leaves.

Indexed as

Fungal ProteinsMembrane ProteinsPlant DiseasesPlant LeavesUstilagoZea maysBasidiomycotaFungal ProteinsMembrane ProteinsAntibody-based detectionCell-wall-associated proteinEffectorsImmunolocalizationMaizeUstilago maydis

Identifiers

PMID42681052

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.