ArticleMethods in molecular biology (Clifton, N.J.)2026
Determination of Spontaneous Mutation Rate by Fluctuation Analysis Using 5-Fluoroorotic Acid (5-FOA).
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Spontaneous mutations serve as critical indicators of genome stability. Regardless of the mechanism, the rate of mutation in a certain genetic background can often provide insight into the underlying biological processes that impact the accuracy of DNA replication or the efficacy of DNA repair. A common method for estimating mutation rates is the Luria-Delbrück fluctuation assay. Here, a protocol is presented for determining mutation rates in U. maydis by fluctuation analysis, using 5-fluoroorotic acid (5-FOA) as a selective agent. The protocol starts with a number of small parallel inoculums of a given strains, which are then grown to high-density cultures. Subsequently, the cultures are screened for mutants by plating onto solid medium containing 5-FOA. The median numbers of 5-FOA-resistant mutants observed on the plates are used to calculate the mutation rates.
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