Evidence map›Paper›PMID 42681043›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Efficient Introduction of Genetic Elements at Specific Insertion Loci.

Sainath Shanmugasundaram, Lasse van Wijlick, Matias D Zurbriggen, Michael Feldbrügge, Kerstin Schipper

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Sainath ShanmugasundaramInstitute of Microbiology, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
Lasse van WijlickInstitute of Microbiology, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
Matias D ZurbriggenInstitute of Synthetic Biology and CEPLAS-Cluster of Excellence on Plant Sciences, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
Michael FeldbrüggeInstitute of Microbiology, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
Kerstin SchipperInstitute of Microbiology, Heinrich Heine University Düsseldorf, Düsseldorf, Germany. kerstin.schipper@hhu.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

While some genetic modifications are conducted at the native loci of the manipulated genes, "neutral" loci for insertion of (heterologous) genetic elements are equally important for a versatile model system. This, e.g., applies for insertion of genetic complementation constructs or for transplantation of heterologous metabolic pathways. Here, the most frequently used insertion loci of Ustilago maydis is presented, namely the so-called ip locus and three loci encoding secreted proteases like the upp3 locus. The strategy of counter selection provides additional benefits in strain generation as it significantly speeds up the identification of correct mutants. Considerations and strategies for construct design and strain generation based on published standard protocols are provided, followed by a detailed example for upp3 locus insertion combined with counter selection. In essence, these versatile strategies for genetic manipulation have the power to simplify and speed up genetic engineering significantly.

Indexed as

Genetic EngineeringGenetic LociMutagenesis, InsertionalUstilagoCounter-selectionInsertion locusIron-sulfur proteinProteaseTranscriptional unit

Identifiers

PMID42681043

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.