Evidence map›Paper›PMID 42681041›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

FLP/FRT-Mediated Recombination for Resistance Marker Recycling.

Lorenzo García Tejada, An N T Phan, Kerstin Schipper, Lars M Blank

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Lorenzo García TejadaInstitute of Applied Microbiology-iAMB, Aachen Biology and Biotechnology-ABBt, RWTH Aachen University, Aachen, Germany.
An N T PhanInstitute of Applied Microbiology-iAMB, Aachen Biology and Biotechnology-ABBt, RWTH Aachen University, Aachen, Germany.
Kerstin SchipperInstitute of Microbiology, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
Lars M BlankWSS Research Centre "Catalaix", Aachen, Germany. Lars.Blank@rwth-aachen.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The availability of selection markers for metabolic engineering of Ustilago species is limited. The inducible flippase (FLP) recombinase system allows for the recycling of a selection marker after a successful recombination event. Following a traditional marker-based homologous recombination of DNA for deletion or insertion of a gene using special constructs with two flanking Flippase-Recognition Targets (FRT) at both sides of the selection marker, FLP-mediated recombination allows for marker removal after transformation. This enables its recycling for a further transformation approach. In Ustilago maydis, the FLP protein has been codon optimized and configured under the control of an arabinose-inducible promoter in a self-replicating plasmid for episomal expression of the recombinase. This FLP/FRT system facilitates advanced genetic modifications in Ustilago spp. by enabling repeated genetic manipulation with one marker, thus streamlining molecular genetic methodologies.

Indexed as

DNA NucleotidyltransferasesNucleotidyltransferasesRecombination, GeneticUstilagoBasidiomycotaGenetic MarkersHomologous RecombinationMetabolic EngineeringPlasmidsDNA NucleotidyltransferasesFLP recombinaseGenetic MarkersNucleotidyltransferasesAntibiotic marker recyclingFlippaseFLP/FRTGene deletionRecombination

Identifiers

PMID42681041

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.