Evidence map›Paper›PMID 42681039›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Golden Gate Assembly for Genome Editing in Ustilago maydis.

Andreas Müsgens, An N T Phan, Kerstin Schipper, Lars M Blank

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Andreas MüsgensInstitute of Applied Microbiology-iAMB, Aachen Biology and Biotechnology-ABBt, RWTH Aachen University, Aachen, Germany.
An N T PhanInstitute of Applied Microbiology-iAMB, Aachen Biology and Biotechnology-ABBt, RWTH Aachen University, Aachen, Germany.
Kerstin SchipperInstitute of Microbiology, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
Lars M BlankInstitute of Applied Microbiology-iAMB, Aachen Biology and Biotechnology-ABBt, RWTH Aachen University, Aachen, Germany. lars.blank@rwth-aachen.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Golden Gate cloning has become a key strategy for genetic manipulation of Ustilago maydis. Originally established for this organism by Terfrüchte et al. (2014), the approach uses type IIS restriction enzyme BsaI to assemble homology arms and integration module in a single reaction, yielding a plasmid from which a linear DNA fragment for transformation can be excised. In this chapter, the Golden Gate method for U. maydis is presented, with a focus on primer design and the DNA assembly workflow, taking practical considerations into account. By structuring the method into a step-by-step guide, it is made accessible for researchers new to U. maydis aiming to generate genome engineered mutants in this intriguing fungus.

Indexed as

Gene EditingGenome, FungalUstilagoBasidiomycotaCloning, MolecularPlasmidsBsaIGene deletionGenomic modificationsGolden Gate cloningType IIS restriction enzymeUstilago maydis

Identifiers

PMID42681039

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.