Evidence map›Paper›PMID 42681038›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Southern Blotting: A Valuable Molecular Technique to Verify Gene Replacement in Ustilago maydis.

Shivam Chaudhary, Lukas Dorian Dittiger, Jan Schirawski

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Shivam ChaudharyMatthias Schleiden Institute/Genetics, Friedrich Schiller University Jena, Jena, Germany.ORCID http://orcid.org/0009-0001-6452-2057
Lukas Dorian DittigerMatthias Schleiden Institute/Genetics, Friedrich Schiller University Jena, Jena, Germany.ORCID http://orcid.org/0000-0001-7640-2661
Jan SchirawskiMatthias Schleiden Institute/Genetics, Friedrich Schiller University Jena, Jena, Germany. jan.schirawski@uni-jena.de.ORCID http://orcid.org/0000-0003-1615-1201

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

One of the most often applied procedures in the quest for gene function analysis in Ustilago maydis is to replace the target gene with an antibiotic resistance cassette by double homologous recombination with a linear gene replacement construct. Unfortunately, many unwanted events can also happen, which would go unnoticed if relying on PCR to verify the resulting strains. Therefore, it is recommended to verify the proper gene replacement using the easily doable and highly informative Southern blotting technique. This chapter provides a detailed and robust protocol for the verification of proper gene replacement in U. maydis mutants by non-radioactive Southern blotting.

Indexed as

Blotting, SouthernUstilagoBasidiomycotaHomologous RecombinationPolymerase Chain ReactionAgarose gelCapillary blotDigoxigeninGene replacementGenomic DNANon-radioactiveNylon membraneSouthern blottingUstilago maydis

Identifiers

PMID42681038

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.