Evidence map›Paper›PMID 42681036›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Isolation of Genomic DNA from Ustilago Species.

Felicia Zlati, Andreas Müsgens, Lars M Blank, Nick Wierckx, Kerstin Schipper

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Felicia ZlatiInstitute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich, Jülich, Germany.
Andreas MüsgensInstitute of Applied Microbiology-iAMB, Aachen Biology and Biotechnology-ABBt, RWTH Aachen University, Aachen, Germany.
Lars M BlankInstitute of Applied Microbiology-iAMB, Aachen Biology and Biotechnology-ABBt, RWTH Aachen University, Aachen, Germany.
Nick WierckxInstitute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich, Jülich, Germany. n.wierckx@fz-juelich.de.
Kerstin SchipperInstitute of Microbiology, Heinrich Heine University Düsseldorf, Düsseldorf, Germany. kerstin.schipper@hhu.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reliable genomic DNA isolation is essential for investigating the genetic basis of any organism. In fungi such as Ustilago maydis, this task is particularly challenging due to the rigid cell wall and the presence of polysaccharides, pigments, and other compounds that can compromise DNA quality as contaminants. This chapter provides practical guidance on overcoming these challenges by detailing robust cell lysis strategies, including enzymatic digestion and mechanical disruption, which form the critical first step in gDNA extraction. Additionally, a set of protocols is presented, tailored to different downstream applications, from PCR amplification to high-throughput short- and long-read sequencing for complex genome assemblies.

Indexed as

DNA, FungalGenome, FungalGenomicsUstilagoBasidiomycotaHigh-Throughput Nucleotide SequencingPolymerase Chain ReactionSequence Analysis, DNADNA, FungalCell lysisEnzymatic digestionGenomic DNAHMW gDNAMechanical disruptionPCRSequencingUstilago

Identifiers

PMID42681036

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.