Evidence map›Paper›PMID 42681035›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

U. maydis Protoplast Generation and Transformation.

Hariharan V Chinnasamy, Gouri S Nair, Saravanan Matheshwaran

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Hariharan V ChinnasamyMicrobial Adaptation and Chromatin Remodeling Laboratory, Department of Biological Sciences and Bioengineering, Environmental Microbiology Laboratory, Center for Environmental Sciences and Engineering, Indian Institute of Technology, Kanpur, Uttar Pradesh, India.
Gouri S NairMicrobial Adaptation and Chromatin Remodeling Laboratory, Department of Biological Sciences and Bioengineering, Environmental Microbiology Laboratory, Center for Environmental Sciences and Engineering, Indian Institute of Technology, Kanpur, Uttar Pradesh, India.
Saravanan MatheshwaranMicrobial Adaptation and Chromatin Remodeling Laboratory, Department of Biological Sciences and Bioengineering, Environmental Microbiology Laboratory, Center for Environmental Sciences and Engineering, Indian Institute of Technology, Kanpur, Uttar Pradesh, India. saran@iitk.ac.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ustilago maydis is a dimorphic basidiomycete that exists as a yeast-like haploid form and a filamentous dikaryotic form. The yeast-like morphology is exploited for protoplast-based, PEG (polyethylene glycol)-mediated transformation, which is a simple and reliable means for introducing DNA into protoplasts. In U. maydis, this approach has been widely used for gene deletion/insertion, complementation, and transgene expression. In short, U. maydis yeast-like cells are grown to mid-log phase, and their cell walls are enzymatically digested in the presence of an osmotic stabilizer to generate stable protoplasts. Exogenous naked DNA, usually a linear integration construct with antibiotic resistance cassette or any other selection marker, is incubated with protoplasts, followed by the addition of high-molecular-weight PEG to enable DNA uptake. In this chapter, we point out the theoretical basis and outline of the procedure.

Indexed as

ProtoplastsTransformation, GeneticUstilagoBasidiomycotaPolyethylene GlycolsPolyethylene GlycolsFungal geneticsOsmotic stabilizationPolyethylene glycol (PEG)ProtoplastSCS bufferSTC bufferTransformationUstilago maydisVinoflow

Identifiers

PMID42681035

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.