Evidence map›Paper›PMID 42681029›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Apoplastic Fluid Collection/Analysis of Ustilago maydis-Infected Maize Leaves.

Yu-Han Lin, Chibbhi Bhaskar, Lay-Sun Ma

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Yu-Han LinInstitute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
Chibbhi BhaskarInstitute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
Lay-Sun MaInstitute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan. laysunma@as.edu.tw.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter presents a detailed protocol for collecting and analyzing apoplastic-localized proteins from Ustilago maydis-infected maize leaves, using vacuum infiltration followed by centrifugation. It is suitable for protein profiling and the immunoblot detection of secreted effectors, providing a valuable tool for investigating host-pathogen interactions in the apoplast and offering insights into how U. maydis modulates this extracellular space during infection.

Indexed as

Fungal ProteinsPlant DiseasesPlant LeavesUstilagoZea maysBasidiomycotaHost-Pathogen InteractionsPlant ProteinsFungal ProteinsPlant ProteinsApoplastic fluid extractionApoplastic-localized effectorsTrichloroacetic acid precipitationU. maydis-infected maizeVacuum infiltration–centrifugation

Identifiers

PMID42681029

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.