Evidence map›Paper›PMID 42681028›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Ustilago maydis Seedling Infection Assay and Scoring.

Patrice Julian Zsoldos, Ziyang Chen, Armin Djamei

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Patrice Julian ZsoldosDepartment of Plant Pathology, Institute of Crop Science and Resource Conservation (INRES), University of Bonn, Bonn, Germany.
Ziyang ChenDepartment of Plant Pathology, Institute of Crop Science and Resource Conservation (INRES), University of Bonn, Bonn, Germany.
Armin DjameiDepartment of Plant Pathology, Institute of Crop Science and Resource Conservation (INRES), University of Bonn, Bonn, Germany. djamei@uni-bonn.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This protocol outlines a reproducible method for assessing the virulence of Ustilago maydis strains in Zea mays seedlings through targeted syringe inoculation of the leaf whorl. Using seedlings at the V3-V4 growth stage ensures optimal susceptibility and developmental uniformity. The method can be used with both solopathogenic strains and compatible mating-type combinations, enabling comparative analyses of infection efficiency and symptom development. Fungal cultures are prepared under controlled conditions to maintain virulence, and post-infection humidity is regulated to enhance colonization success. Symptom progression is monitored over 10-12 days and quantified using an ordinal disease scoring system. This assay provides a robust tool for evaluating effector mutants, analyzing host-pathogen interactions, and comparing strain virulence under standardized conditions.

Indexed as

Plant DiseasesSeedlingsUstilagoZea maysBasidiomycotaHost-Pathogen InteractionsPlant LeavesVirulenceDisease scoringFungal virulenceLeaf whorl infectionMaize smutPathogenicity assayplant–microbe interactionSeedling infection protocolUstilago maydis

Identifiers

PMID42681028

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.