Evidence map›Paper›PMID 42681022›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Posttranslational Dynamics of the Subcellular Proteome by Mass Spectrometry-Based Proteomics.

Ana Martínez-Val, Jose Manuel Rodríguez, Jesper V Olsen, Jesús Vázquez

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Ana Martínez-ValCardiovascular Proteomics Laboratory, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain. ana.martinezdelval@cnic.es.
Jose Manuel RodríguezCardiovascular Proteomics Laboratory, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain.
Jesper V OlsenNovo Nordisk Foundation Center for Protein Research, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Jesús VázquezCardiovascular Proteomics Laboratory, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Localization of proteins to different cell compartments is a major posttranslational regulatory mechanism that eukaryotic cells have evolved to coordinate protein homeostasis and responses to stimuli. Subcellular fractionation allows the separation of distinct protein populations from cellular compartments such as the cytosol, cytoskeleton, membrane, endoplasmic reticulum, or nucleolus. This fractionation can be followed by mass spectrometry-based proteomics to provide insights into spatiotemporal regulation or cellular protein networks. Here, we describe a high-throughput workflow based on sequential cell lysis, which enables the profiling of subcellular proteome architecture and the detection of changes in protein localization at global and posttranslational levels.

Indexed as

Mass SpectrometryProtein Processing, Post-TranslationalProteomeProteomicsAnimalsCell FractionationHumansSubcellular FractionsProteomeCell signallingMass spectrometryPosttranslational regulationProteomicsSubcellular fractionation

Identifiers

PMID42681022

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.