Evidence map›Paper›PMID 42681011›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Reconstituting the Motility of Intraflagellar Transport Trains Ex Vivo.

Aditya Chhatre, Ludek Stepanek, Stefan Diez, Gaia Pigino

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Aditya ChhatreBiotechnology Center, TUD Dresden University of Technology, Dresden, Germany.
Ludek StepanekInstitute of Molecular Genetics, Czech Academy of Sciences, Prague, Czech Republic.
Stefan DiezCluster of Excellence Physics of Life, TUD Dresden University of Technology, Dresden, Germany. stefan.diez@tu-dresden.de.
Gaia PiginoHuman Technopole, Milan, Italy. gaia.pigino@fht.org.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Intraflagellar transport (IFT) is essential for the assembly, maintenance and function of most eukaryotic cilia. It acts as a bidirectional cargo transport system, powered by kinesin and dynein motors to navigate through the crowded ciliary environment. Here, we describe a method to isolate intact IFT trains from live cells and reconstitute their motility on synthetic microtubules in vitro. This system enables the controlled investigation of the biophysical properties of IFT trains, including their sensitivity to tubulin post-translational modifications. With minor adaptations, this approach can be extended to study other intracellular transport processes.

Indexed as

CiliaFlagellaAnimalsBiological TransportDyneinsKinesinsMicrotubulesTubulinDyneinsKinesinsTubulinCiliaDyneinEx vivo single cell assayIntraflagellar transportIn vitro motility assayKinesinMicrotubules

Identifiers

PMID42681011

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.