Lighting Up Biology: GFP-Inspired Fluorescent Probes for Sensing and Imaging.

Qianfang Qiu, Luling Wu, Robin R Groleau, Xuanyu Zhu, Tony D James, Chusen Huang

Abstract read

In one paragraph

Article in Journal of the American Chemical Society, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Qianfang QiuThe Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Frontiers Science Center of Biomimetic Catalysis, and Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, Department of Chemistry, Shanghai Normal University, 100 Guilin Road, Shanghai200234, China.

Luling WuState Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry, Nanjing University, 163 Xianlin Avenue, Nanjing210023, China.ORCID 0000-0001-6574-5861

Robin R GroleauDepartment of Life Sciences, University of Bath, BathBA2 7AY, U.K.ORCID 0000-0003-0213-5198

Xuanyu ZhuThe Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Frontiers Science Center of Biomimetic Catalysis, and Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, Department of Chemistry, Shanghai Normal University, 100 Guilin Road, Shanghai200234, China.

Tony D JamesDepartment of Chemistry, University of Bath, BathBA2 7AY, U.K.ORCID 0000-0002-4095-2191

Chusen HuangThe Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Frontiers Science Center of Biomimetic Catalysis, and Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, Department of Chemistry, Shanghai Normal University, 100 Guilin Road, Shanghai200234, China.ORCID 0000-0002-2481-9147

Funding

Henan Normal University 2020ZD01Key Laboratory of Bioanalysis and Metrology for State Market Regulation A04202423005Natural Science Foundation of Shanghai 25ZR1401275Shanghai Engineering Research Center of Green Energy Chemical Engineering NAShanghai Municipal Education Commission NAShanghai Municipal People's Government 18DZ2254200Shanghai Normal University NAUniversity of Bath NA

6 · The paper itself

Abstract

Green fluorescent protein (GFP) chromophore analogues are derived from the core structure of the GFP chromophore, p-hydroxybenzylideneimidazolidinone (HBDI). Appealing and readily tunable, HBDI analogues have been adapted for a range of sensing and imaging applications, as is the focus of this perspective. First, common methods for their synthesis will be presented, followed by a discussion of the unique structural and photophysical properties of HBDI derivatives. Structural modifications resulting in tunable photophysical properties will then be highlighted. Finally, this perspective will present representative examples of the use of HBDI analogues for sensing and imaging in biological sciences. This perspective highlights key design strategies, modes of activation, and the associated advantages, limitations, and opportunities of HBDI analogues, illustrated by representative applications in studying biomolecules, cellular processes, and disease biomarkers.

Indexed as

Fluorescent DyesGreen Fluorescent ProteinsOptical ImagingAnimalsFluorescent Chemosensor CompoundsHumansFluorescent Chemosensor CompoundsFluorescent DyesGreen Fluorescent Proteins

Identifiers

PMID42677517

DOI10.1021/jacs.5c16404

PMCPMC13523761

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.