ArticleJournal of tropical medicine2026
Molecular Characterization of DENV-1 Isolates From Pakistan Reveals Immunogenic Variability and Antiviral Potential of Phytochemicals Targeting the CprM Region.
Article in Journal of tropical medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Molecular Characterization of DENV-1 Isolates From Pakistan Reveals Immunogenic Variability and Antiviral Potential of Phytochemicals Targeting the CprM Region.Journal of tropical medicine · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dengue virus (DENV), a mosquito-borne arbovirus, remains a critical global health concern, causing approximately 390 million infections annually. Dengue fever poses significant challenges to healthcare systems in endemic regions like Pakistan due to the presence of four established serotypes (DENV-1-DENV-4) and the potential emergence of a fifth serotype (DENV-5). The current study aimed to investigate molecular characteristics of DENV-1 isolates from Peshawar, Pakistan, focusing on serotyping, genetic variability in the CprM junction region, and identification of potential antiviral phytochemicals. A total of 45 patient samples were screened, with the highest infection rate (49%) observed among individuals aged 20-40 years. Phylogenetic analysis revealed that six isolates clustered within the DENV-1 clade and grouped closely related to isolates from China, India, Bangladesh, and Pakistan, suggesting a regional evolution and potentially cross-border transmission. The prominent amino acid substitutions were Q47H, G48E, P49H, K51 E/N, L52 M, and M57I, which may influence viral structure, immune evasion, and antigenic properties. To address the lack of effective antivirals, a library of 60 phytochemicals was screened. Nine compounds demonstrated favorable ADMET profiles and high binding affinity (docking scores < -100) with the CprM region. Silydianin exhibited the strongest interaction (-159.81), indicating promising inhibitory potential. This integrative molecular and computational approach enhances our understanding of circulating DENV-1 strains and highlights plant-derived compounds as candidates for future antiviral development, pending in vitro validation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.