Evidence map›Paper›PMID 42673761›Full record

ArticleEBioMedicine2026

Genomic and transcriptomic features of HBV integration in treatment-naïve, HBeAg-positive children with chronic HBV infection.

Na Wu, Yan Han, Jing Tang, Zhiwei Chen, Jiaying Wu, Yingzhi Zhou, Yunan Chang, Xiaorong Peng, Aoxue Tan, Yi Xiang and 14 more

Abstract read
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Article in EBioMedicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

24 authors.

Na WuDepartment of Infectious Diseases, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China; Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Yan HanDepartment of Infectious Diseases, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China; Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Jing TangDepartment of Infectious Diseases, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China; Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Zhiwei ChenDepartment of Infectious Diseases, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China; Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Jiaying WuDepartment of Infectious Diseases, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Child Infection and Immunity, Chongqing, China.
Yingzhi ZhouKey Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China; Department of Infectious Diseases, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Child Infection and Immunity, Chongqing, China.
Yunan ChangDepartment of Infectious Diseases, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Child Infection and Immunity, Chongqing, China.
Xiaorong PengDepartment of Infectious Diseases, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Child Infection and Immunity, Chongqing, China.
Aoxue TanDepartment of Infectious Diseases, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Child Infection and Immunity, Chongqing, China.
Yi XiangKey Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China; Department of Hepatology, Chongqing University Three Gorges Hospital, Chongqing, China.
Fang WeiKey Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China; Department of Hepatology, Chongqing University Three Gorges Hospital, Chongqing, China.
Guanhua ZhaDepartment of Infectious Diseases, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China; Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Zhiling DengDepartment of Infectious Diseases, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China; Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Qingliang WangDepartment of Pathology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Li ZhangDepartment of Infectious Diseases, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Xiaohao WangDepartment of Infectious Diseases, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Wei ShenKey Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Peng HuDepartment of Infectious Diseases, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China; Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Mingli PengKey Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Dachuan CaiDepartment of Infectious Diseases, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China; Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Xinxin ZhangDepartment of Infectious Diseases, Research Laboratory of Clinical Virology, Shanghai Jiao Tong University Medical School Affiliated Ruijin Hospital, Shanghai, China. Electronic address: zhangx@shsmu.edu.cn.
Xuan AnDepartment of Hepatology, Chongqing University Three Gorges Hospital, Chongqing, China. Electronic address: anxuan@cqu.edu.cn.
Hongmei XuDepartment of Infectious Diseases, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Child Infection and Immunity, Chongqing, China. Electronic address: xuhongm0095@cqmu.edu.cn.
Hong RenDepartment of Infectious Diseases, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China; Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China. Electronic address: renhong0531@cqmu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHepatitis B virus (HBV) integration represents a major obstacle to curing HBV; however, the landscape of HBV integration and local immune response to transcriptionally active viral integration in children with chronic HBV infection remain unclear. Herein, we aimed to elucidate this landscape in this population.

methodsGenomic analyses using a probe-based capture strategy were performed on 18 children and 28 adults with chronic HBV infection. Spatial transcriptomics (ST) was performed on 12 children from our cohort and 3 adults from a public database.

findingsAll patients were hepatitis B e antigen (HBeAg)-positive and treatment-naïve. Genomically, children exhibited significantly lower clonal expansion level of HBV-integrated hepatocytes than adults, despite comparable unique breakpoint counts. After adjusting for confounding variables, age was identified as an independent risk factor for total frequency of unique integration breakpoints (b = 3.22, P = 0.005). Spatially, ST revealed that spots with transcriptionally active viral integration exhibited a sparse distribution and accounted for a low proportion of all spots in children. Notably, at these spots, children showed reduced adaptive immune cells (e.g., CD8

interpretationCompared with adults, children exhibit lower clonal expansion of HBV-integrated hepatocytes and distinct immune profiles in response to transcriptionally active viral integration, offering new insights into their differing clinical course.

fundingKey Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education).

Indexed as

GenomicsHepatitis B, ChronicHepatitis B e AntigensHepatitis B virusTranscriptomeVirus IntegrationAdolescentAdultChildChild, PreschoolFemaleGene Expression ProfilingHepatocytesHumansMaleMiddle AgedHepatitis B e AntigensChildHepatitis B virusIntegrationSpatial transcriptomics

Identifiers

PMID42673761
PMCPMC13562084

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