Evidence map›Paper›PMID 42673434›Full record

ArticleChembiochem : a European journal of chemical biology2026

Unnatural C6-Substituted d-Galactosides as Attenuated Substrates of Glycosyl Hydrolases.

Andrey Yu Plodukhin, Karel Hernández, Suada Demirovic, Taiwo Ogunmilade, Brandon Q Mercado, Meghan Kennedy, Alfredo Angeles-Boza, Antoni Planas, Mark W Peczuh

Abstract read
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In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Andrey Yu PlodukhinDepartment of Chemistry, University of Connecticut, Storrs, Connecticut, USA.ORCID https://orcid.org/0000-0002-6830-1155
Karel HernándezLaboratory of Biochemistry, Institut Químic de Sarrià, University Ramon Llull, Barcelona, Spain.ORCID https://orcid.org/0000-0001-7367-4426
Suada DemirovicDepartment of Chemistry, University of Connecticut, Storrs, Connecticut, USA.ORCID https://orcid.org/0009-0002-6632-8183
Taiwo OgunmiladeDepartment of Chemistry, University of Connecticut, Storrs, Connecticut, USA.ORCID https://orcid.org/0009-0002-5476-6768
Brandon Q MercadoChemical and Biophysical Instrumentation Center, Yale University, New Haven, Connecticut, USA.ORCID https://orcid.org/0000-0002-1940-3497
Meghan KennedyDepartment of Chemistry, University of Connecticut, Storrs, Connecticut, USA.ORCID https://orcid.org/0009-0001-1666-3154
Alfredo Angeles-BozaDepartment of Chemistry, University of Connecticut, Storrs, Connecticut, USA.ORCID https://orcid.org/0000-0002-5560-4405
Antoni PlanasLaboratory of Biochemistry, Institut Químic de Sarrià, University Ramon Llull, Barcelona, Spain.ORCID https://orcid.org/0000-0001-7073-3320
Mark W PeczuhDepartment of Chemistry, University of Connecticut, Storrs, Connecticut, USA.ORCID https://orcid.org/0000-0002-3028-6551

Funding

MINECO PID2022-138252OB-I00National Science Foundation CHE-2320586
6 · The paper itself

Abstract

The covalent attachment of pendant groups to the carbons of d-pyranosides can influence their ability to be recognized by proteins that bind natural sugars. These carbon-modified sugars have received only limited attention, and systematic investigations on their ability to act as substrates of glycosyl hydrolases have not been reported. Here, C6-substituted β-d-galactosides are synthesized and evaluated as putative substrates of β-glycosyl hydrolases. The results indicated that the size of the group at C6-methyl vs. di-methyl vs. phenyl-attenuated their ability to act as substrates. The C6-methyl derivative was a weak substrate, but the C6-dimethyl and C6-phenyl compounds were not. The new β-galactosides can be leveraged as tool compounds in the search for glycosidases with novel activity and as starting points for enzyme-engineering campaigns.

Indexed as

GalactosidesGlycoside HydrolasesSubstrate SpecificityGalactosidesGlycoside Hydrolasesbioorthogonalcarbon‐modified sugargalactoseglycomimeticglycosidase

Identifiers

PMID42673434
PMCPMC13529265

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.