Evidence map›Paper›PMID 42671580›Full record

ArticleVirchows Archiv : an international journal of pathology2026

Design and analytical validation of Nexthyro 2.0, a custom next-generation sequencing panel integrating DNA mutation profiling and RNA fusion detection for routine thyroid pathology specimens.

Mariantonia Nacchio, Anna Maria Carillo, Domenica Di Giovanni, Genevieve Belleannee, Beatrix Cochand-Priollet, Myriam Decaussin-Petrucci, Gabrielle Deniziaut, Hélène Trouette, Philippe Vielh, Elena Vigliar and 3 more

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Article in Virchows Archiv : an international journal of pathology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

13 authors.

Mariantonia Nacchio *Department of Public Health, Pathology Division, University of Naples Federico II, Naples, Italy.ORCID http://orcid.org/0000-0002-8528-5273
Anna Maria Carillo *Department of Public Health, Pathology Division, University of Naples Federico II, Naples, Italy.
Domenica Di Giovanni *Department of Public Health, Pathology Division, University of Naples Federico II, Naples, Italy.
Genevieve BelleanneeHôpital du Haut-Lévêque, CHU de Bordeaux, Pessac, 33604, France.
Beatrix Cochand-PriolletDepartment of Pathology, Cochin Hospital, Paris, France.
Myriam Decaussin-PetrucciService d'anatomie pathologique, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, Université Lyon 1, Pierre Bénite, 3738, EA, France.ORCID http://orcid.org/0000-0002-6065-0756
Gabrielle DeniziautDepartment of Anatomopathology, AP-HP, Hôpital Pitié-Salpêtrière, Paris, France.
Hélène TrouetteAnatomopathology, Bordeaux University Hospital, Bordeaux, France.
Philippe VielhDepartment of Pathology, Medipath and the American Hospital of Paris, Paris, France.ORCID http://orcid.org/0000-0003-2574-3241
Elena VigliarDepartment of Public Health, Pathology Division, University of Naples Federico II, Naples, Italy.ORCID http://orcid.org/0000-0003-2856-9023
Umberto MalapelleDepartment of Public Health, Pathology Division, University of Naples Federico II, Naples, Italy.ORCID http://orcid.org/0000-0003-3211-9957
Giancarlo TronconeDepartment of Public Health, Pathology Division, University of Naples Federico II, Naples, Italy. giancarlo.troncone@unina.it.ORCID http://orcid.org/0000-0003-1630-5805
Claudio BellevicineDepartment of Public Health, Pathology Division, University of Naples Federico II, Naples, Italy.ORCID http://orcid.org/0000-0002-7479-6457

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The increasing role of molecular testing in thyroid pathology, from the pre-operative stratification of indeterminate fine-needle aspiration (FNA) categories to the management of advanced carcinomas, has prompted the development of institution-specific custom NGS solutions as a locally deployable alternative to centralized tests. The aim of the present study is to validate a new custom NGS panel dedicated to the molecular analysis of routine thyroid pathology specimens. The panel was evaluated on thyroid cancer cell lines, 124 FFPE thyroid tissue specimens, and 43 FNA samples. Nexthyro 2.0 achieved a high sequencing success rate on both histological (100%) and cytological (95.3%) material, with analytical sensitivity down to 0.5% dilution point. Mutational profiles across thyroid neoplasm histotypes and FNA categories were consistent with established molecular-morphological correlates. Nexthyro 2.0 represents a reliable local solution for thyroid molecular testing across cytological and histological specimens encountered in routine clinical practice.

Indexed as

Analytical validationFine-needle aspirationMolecular diagnosticsNext-generation sequencingThyroid cytology

Identifiers

PMID42671580

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