Evidence map›Paper›PMID 42671480›Full record

ArticleHuman reproduction (Oxford, England)2026

A Western-style diet exacerbates male infertility in M1ap knockout mice.

Brendan J Houston, Joseph Nguyen, Nadja Rotte, Frank Tüttelmann, Corinna Friedrich, D Jo Merriner, Anne E O'Connor, Moira K O'Bryan, Jessica E M Dunleavy

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Article in Human reproduction (Oxford, England), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Brendan J HoustonSchool of BioSciences and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia.ORCID 0000-0002-1078-756X
Joseph NguyenSchool of BioSciences and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia.
Nadja RotteInstitute of Reproductive Genetics, Centre of Medical Genetics, University of Münster, Münster, Germany.
Frank TüttelmannInstitute of Reproductive Genetics, Centre of Medical Genetics, University of Münster, Münster, Germany.ORCID 0000-0003-2745-9965
Corinna FriedrichInstitute of Reproductive Genetics, Centre of Medical Genetics, University of Münster, Münster, Germany.
D Jo MerrinerSchool of BioSciences and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia.
Anne E O'ConnorSchool of BioSciences and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia.
Moira K O'BryanSchool of BioSciences and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia.ORCID 0000-0001-7298-4940
Jessica E M DunleavySchool of BioSciences and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia.ORCID 0000-0003-2009-0165

Funding

Australia-Germany Joint Research Co-operation Scheme 57511796Clinical Research Unit 'Male Germ Cells' 329621271Deutsche ForschungsgemeinschaftDFG, German Research FoundationNational Health and Medical Research Council APP1120356
6 · The paper itself

Abstract

study questionWhat are the individual and combined effects of a common genetic cause of human male infertility (loss of M1AP function) and a negative lifestyle factor (a Western diet) on male fertility, spermatogenesis, and sperm quality in mice? SUMMARY ANSWER: The loss of M1AP and a Western diet separately led to subfertility (a form infertility) in male mice and in combination subfertility was worsened. WHAT IS KNOWN ALREADY: Genetics and lifestyle factors individually play important roles in male infertility aetiology. How lifestyle factors influence common genetic causes of infertility are unknown. STUDY DESIGN, SIZE, DURATION: We generated knockout M1ap mice (M1ap-/--) and investigated the effects of genotype (wild type or knockout) and diet (control or Western diet) or genotype and age (young or aged) on male fertility and sperm parameters. PARTICIPANTS/MATERIALS, SETTINGS,

methodsC57BL6/J mice: wild type (M1ap+/+) and M1ap knockout (M1ap-/-) at 10-12 (young) weeks or 26-28 weeks old (aged), on a control of Western (high-fat and high-sugar) diet and age. We evaluated male fertility, testis and epididymis histology, and sperm quality (motility, morphology, and count). MAIN RESULTS AND THE ROLE OF CHANCE: Compared to the individual gene deletion or diet treatments, the combined M1ap-/- and Western diet group had more abnormal germ cells, sloughed germ cells and resulted in fewer pups per litter. In accordance, sperm motility and morphology in the combination group were more significantly compromised compared to individual experimental groups. LARGE SCALE DATA: N/A. LIMITATIONS, REASONS FOR CAUTION: While the mutant mouse mimics the genetic variants seen in many infertile men, this is a mouse model study, meaning findings should be validated in a human cohort where possible. WIDER IMPLICATIONS OF THE

findingsTaken together, our data highlight that genetic and lifestyle factors can interact to exacerbate male infertility. This study sets a precedent for an improved consideration of how genetic, lifestyle and environmental factors contribute to infertility aetiology. These data inform a clinical foundation for lifestyle interventions for men presenting at infertility clinics prior to medically assisted reproduction.

fundingThis project was supported by a Society for Reproductive Biology Career Development Award to B.J.H., National Health and Medical Research Council project grant (APP1120356) awarded to M.K.O.B., an Australia-Germany Joint Research Co-operation Scheme (UA-DAAD) grant (57511796) awarded to M.K.O.B., J.E.M.D., and F.T., and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) within the Clinical Research Unit 'Male Germ Cells' (CRU326, project number 329621271) awarded to F.T. and C.F. DISCLOSURES: N/A.

Indexed as

Diet, WesternInfertility, MaleProstatic Secretory ProteinsAnimalsMaleMiceMice, Inbred C57BLMice, KnockoutSpermatogenesisSpermatozoaSperm MotilityTestisProstatic Secretory Proteinsgenetic variantsM1APmale infertilitysperm qualityWestern diet

Identifiers

PMID42671480
PMCPMC13635463

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.