Evidence map›Paper›PMID 42670033›Full record

ArticleTransboundary and emerging diseases2026

Prevalence and Molecular Characterization of FAdV-4 Isolated in Fujian Province, Southeast China During 2023-2025.

Youlin Chen, Ziyue Wang, Dingping Bai, Zhen Chen, Cuiteng Chen, Xi Cai, Guanghua Fu, Chunhe Wan, Yu Huang, Chunhua Zhu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Transboundary and emerging diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Youlin ChenFujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China, faas.cn.
Ziyue WangFujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China, faas.cn.
Dingping BaiFujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, College of Animal Sciences, Fujian Agricultural and Forestry University, Fuzhou 350002, China, fjau.edu.cn.
Zhen ChenFujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China, faas.cn.
Cuiteng ChenFujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China, faas.cn.
Xi CaiFujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China, faas.cn.
Guanghua FuFujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China, faas.cn.ORCID https://orcid.org/0009-0004-9579-9691
Chunhe WanFujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China, faas.cn.ORCID https://orcid.org/0000-0003-2011-5609
Yu HuangFujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China, faas.cn.ORCID https://orcid.org/0000-0003-3654-8898
Chunhua ZhuFujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China, faas.cn.ORCID https://orcid.org/0000-0001-7148-4311

Funding

China Agriculture Research System CARS-41-18Fujian Provincial Public Research Institute of Fundamental Research 2025R10240007Fujian Provincial Public Research Institute of Fundamental Research 2025R1071Research and Technology Program of the Fujian Academy of Agricultural Sciences DWHZ2026-04
6 · The paper itself

Abstract

Fowl adenovirus (FAdV) serotype 4 (FAdV-4) is a significant pathogen causing hepatitis-hydropericardium syndrome (HHS) in poultry, which can cause severe economic losses to the global poultry industry. Molecular epidemiological investigation of FAdV-4 has not been conducted in Fujian province, southeast China. In this study, we collected 219 samples of liver exhibiting similar pathological features of hepatitis or hydropericardium syndrome (HPS)-like clinicopathology changes from Fujian province during 2023-2025, which were analyzed using PCR assays. Further isolation of the virus was performed, and complete genomic sequences of the isolates were obtained through next-generation sequencing technology. Genetic evolution analysis of the gene sequences was conducted to elucidate the prevalence, molecular characteristics, and genetic variations of FAdV-4 in Fujian province. The results indicated that among the 219 clinical samples, 65 tested positive, yielding a positive rate of 29.68% (65/219). Positive samples from broilers were predominantly detected in the 21-120 days old, while those from layers were primarily concentrated in the 200-500 days old. The positive rate of Muscovy ducks infected with FAdV-4 was 4.57%. Ultimately, seven strains of FAdV-4 were isolated and designated as FJ08, FJ09, FJ20, FJ23, FJ33, FJ35, and FJ45. The preliminary challenge assay showed that all seven isolates were highly virulent in 7 days old SPF chickens, resulting in the death of all chickens within 2-3 days, leading to a mortality rate of 100%. Comparative analysis of whole genome sequences revealed that the isolated FAdV-4 strains from Fujian province clustered within the major genetic lineage as the domestic FAdV-4 reference strains (e.g., JSJ13, HLJFAd15, and HB1510 strains) and exhibited the highest identity with the FAdV-4 BNC strain derived from black-necked cranes in Yunnan province. Notably, the Hexon genes of seven isolated strains were divided into two major lineages based on phylogenetic analysis. The FJ08, FJ09, and FJ23 strains clustered in the major lineage as representative strains of FAdV-4 from both domestic and international sources, whereas FJ20, FJ33, FJ35, and FJ45 strains formed an independent genetic lineage with the FAdV-10 AHFY19 strain. The prevalence analysis revealed that FAdV-4 has been circulating among commercial chickens, indigenous chicken breeds, and Muscovy ducks in Fujian province, southeast China, indicating that the FAdV-4 strains in Fujian province possess the potential for cross-host transmission. Moreover, the age of the infected hosts (layers) has extended to 500 days, and their genomic characteristics exhibit genetic diversity.

Indexed as

Adenoviridae InfectionsAviadenovirusPoultry DiseasesAnimalsChickensChinaDucksPhylogenyPrevalencefowl adenovirus serotype 4genetic diversitymolecular characterizationprevalencevirus isolation

Identifiers

PMID42670033
PMCPMC13527229

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.