In Vivo Monitoring of Pressure Ulcer Model Development and Recoverability Under Multimodal Parameters Within a Unified Field of View.

This study utilizes an integrated laser speckle (LSCI) and intrinsic optical signal imaging (IOSI) system to evaluate microvascular dynamics in murine pressure ulcer (PU) models. We compared a single prolonged compression (6 h) against repeated compressions (1.5 h/day for 7 days) using an enhanced spatiotemporal gradient analysis of LSCI data. In the repeated compression model, gradient analysis revealed a progressive perfusion decline by Day 4. By Day 7, perfusion gradients indicated near-complete flow loss, corresponding to severe hypoxia detected by IOSI, signifying sustained microvascular impairment. Conversely, the single compression model exhibited reversible alterations; LSCI gradients showed initial disruption followed by gradual recovery, with perfusion nearing baseline by Day 7. The LSCI-IOSI platform, emphasizing advanced gradient processing, systematically delineates microcirculatory compromise. This approach highlights the critical role of repeated ischemia-reperfusion in driving persistent dysfunction and demonstrates the utility of quantitative optical imaging for early detection and targeted intervention in PU progression.