Evidence map›Paper›PMID 42669719›Full record

ArticleVirchows Archiv : an international journal of pathology2026

Multiplex immunofluorescence in the identification of immune cell compositions in rejection, bronchus-associated lymphoid tissue, and infection in lung allograft tissue.

Wipawi Klaisuban, Trynda N Kroneman, Thomas J Flotte, Sarah M Jenkins, Karla J Kopp, Marie-Christine Aubry, Ying-Han R Hsu, Sahar A Saddoughi, John P Scott, Anja C Roden

Abstract read
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Article in Virchows Archiv : an international journal of pathology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Wipawi KlaisubanDepartment of Laboratory Medicine and Pathology, Mayo Clinic Rochester, Hilton 11, 200 First St SW, Rochester, MN, 55905, USA.
Trynda N KronemanDepartment of Laboratory Medicine and Pathology, Mayo Clinic Rochester, Hilton 11, 200 First St SW, Rochester, MN, 55905, USA.
Thomas J FlotteDepartment of Laboratory Medicine and Pathology, Mayo Clinic Rochester, Hilton 11, 200 First St SW, Rochester, MN, 55905, USA.
Sarah M JenkinsDepartment of Quantitative Health Sciences, Mayo Clinic, Rochester, MN, USA.
Karla J KoppDepartment of Laboratory Medicine and Pathology, Mayo Clinic Rochester, Hilton 11, 200 First St SW, Rochester, MN, 55905, USA.
Marie-Christine AubryDepartment of Laboratory Medicine and Pathology, Mayo Clinic Rochester, Hilton 11, 200 First St SW, Rochester, MN, 55905, USA.
Ying-Han R HsuDepartment of Laboratory Medicine and Pathology, Mayo Clinic Rochester, Hilton 11, 200 First St SW, Rochester, MN, 55905, USA.
Sahar A SaddoughiWilliam J. von Liebig Center for Transplantation and Clinical Regeneration Division of Thoracic Surgery & Department of Cardiovascular Surgery, Mayo Clinic, Rochester, MN, USA.
John P ScottDivision of Pulmonary and Critical Medicine, Mayo Clinic, Rochester, MN, USA.
Anja C RodenDepartment of Laboratory Medicine and Pathology, Mayo Clinic Rochester, Hilton 11, 200 First St SW, Rochester, MN, 55905, USA. Roden.anja@mayo.edu.ORCID http://orcid.org/0000-0001-8948-4758

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Spatial immune cell profiling using multiplex immunofluorescence was examined to characterize the inflammatory cell infiltrates and to possibly aid in the distinction of acute cellular rejection (ACR) and lymphocytic bronchiolitis (LB) from its mimickers including bronchus-associated lymphoid tissue (BALT) and infection in lung allograft tissue. Lung allograft biopsies with ACR and/or LB were identified from an institutional pathology lung transplant database (2012-2024). Surgical lung specimens, transbronchial biopsies, and autopsy lungs were used for BALT and infection, respectively. Multiplex immunofluorescence using the PhenoCode Signature Immune Profile Human Protein Panel (CD3e, CD8, CD20, CD68, FoxP3, pankeratin) was performed. The study included tissue specimens with ACR (n = 13), LB (n = 10), BALT (n = 12), and infection (n = 13). The percentage of CD3 + T-cells, CD8 + T-cells, and CD68 + macrophages was higher and the percentage of CD20 + B-cells was lower in ACR, LB, and infection than BALT (p ≤ 0.02, all). The percentage of FoxP3 + CD3 + T-cells was higher in ACR and BALT than infection (p ≤ 0.02, all). In conclusion, ACR and LB are predominantly comprised of T-cells, however B-cells, macrophages, and FoxP3 + CD3 + T-cells are also present. The immune cell composition of ACR and LB is distinct from that of BALT but similar to infection.

Indexed as

Immune cellsLung allograftLung transplantationMultiplex immunofluorescenceRejection

Identifiers

PMID42669719

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.