Evidence map›Paper›PMID 42667463›Full record

ArticleMolecular biology reports2026

Therapeutic effects of 1,25-dihydroxyvitamin D

Shuqi Yan, Haoyan Ma, Wenjian Li, Jiayuan Qu, Ming Wei, Chong Tan, Qizheng Pan, Shuping Ren, Yuhong Wang

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Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Shuqi Yan *Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, 130021, Jilin Province, China.
Haoyan Ma *Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, 130021, Jilin Province, China.
Wenjian Li *Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, 130021, Jilin Province, China.
Jiayuan QuDepartment of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, 130021, Jilin Province, China.
Ming WeiDepartment of Clinical Laboratory of Jilin Provincial Maternal and Child Health Hospital, Jilin Provincial Obstetric Quality Control Center), Changchun, 130061, China.
Chong TanDepartment of Clinical Laboratory of Jilin Provincial Maternal and Child Health Hospital, Jilin Provincial Obstetric Quality Control Center), Changchun, 130061, China.
Qizheng PanDepartment of Anesthesia of the China-Japan Union Hospital of Jilin University, Changchun, 130000, Jilin Province, China.
Shuping RenDepartment of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, 130021, Jilin Province, China.
Yuhong WangDepartment of Clinical Laboratory of Jilin Provincial Maternal and Child Health Hospital, Jilin Provincial Obstetric Quality Control Center), Changchun, 130061, China. 827248118@qq.com.

Funding

Department of Science and Technology of Jilin Province YDZJ202401668ZYTS
6 · The paper itself

Abstract

backgroundApproximately 9 to 26% of pregnant individuals develop gestational diabetes mellitus (GDM), for which insulin resistance is a key driver. 1,25-dihydroxyvitamin D (1,25(OH)₂D₃) and astragalus polysaccharide (APS) can each alleviate insulin resistance, yet their combined mechanisms remain poorly elucidated.

methodsDiabetic rats were induced by high-fat diet and low-dose streptozotocin in Sprague-Dawley rats, then treated with 1,25(OH)₂D₃, APS, or both for two weeks. Serum glucose, insulin, and lipid profiles were measured. In the liver, reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD) activity, liver function markers, and protein expression of vitamin D receptor (VDR), insulin signaling, PI3K/AKT/FOXO1, gluconeogenesis, and lipid metabolism pathways were assessed. Co-immunoprecipitation was also performed to examine VDR-PI3K p85 interaction.

resultsIn GDM rats, combined 1,25(OH)₂D₃ and APS intervention significantly reduced the serum levels of glucose, aspartate aminotransferase, alanine aminotransferase, free fatty acid, triglyceride and apolipoprotein B, and homeostatic model assessment of insulin resistance(p < 0.05). The combination decreased hepatic ROS and MDA levels, increased hepatic SOD activity, upregulated VDR and cytochrome P450 2R1 mRNA levels, and increased the protein expression levels of VDR, insulin receptor, insulin receptor substrate 1, glucose transporter 2, PI3K, AKT, pAKT and the pAKT/AKT ratio (p < 0.05). It also suppressed the protein expression levels of FOXO1, PEPCK, G6pase and SREBP1C, and increased PPARγ levels (p < 0.05). Co-immunoprecipitation assay confirmed a potential interaction between VDR and PI3K p85 in liver tissues.

conclusionCombined 1,25(OH)₂D₃ and APS alleviated insulin resistance and metabolic disorders in GDM rats, possibly via VDR upregulation, oxidative stress attenuation, and PI3K/AKT/FOXO1 signaling modulation.

Indexed as

Astragalus PlantCalcitriolDiabetes, GestationalInsulin ResistancePolysaccharidesVitamin DAnimalsBlood GlucoseDiabetes Mellitus, ExperimentalDiet, High-FatDisease Models, AnimalFemaleForkhead Box Protein O1InsulinLipid MetabolismLiverBlood GlucoseCalcitriolForkhead Box Protein O1Foxo1 protein, ratInsulinPhosphatidylinositol 3-KinasesPolysaccharidesProto-Oncogene Proteins c-aktReactive Oxygen SpeciesReceptors, CalcitriolVitamin DGluconeogenesisHOMA-IRLipid MetabolismOxidative StressPI3K/AKT/FOXO1 PathwayVitamin D Receptor

Identifiers

PMID42667463

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.