Evidence map›Paper›PMID 42666760›Full record

ArticleNeurosurgery practice2026

Integrity of Tumor Tissue Collected by Ultrasonic Aspirator: A Cellular and Molecular Perspective.

Justin Maldonado, Youssef M Zohdy, Karen Salmeron-Moreno, Aliaksandr Aksionau, Masayuki Hirano, Samadhi Senanayake, Razan Faraj, Bethany Chern, Hithardhi Duggireddy, Saikarthik Papisetty and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neurosurgery practice, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Justin MaldonadoDepartment of Neurosurgery, Emory University, Atlanta, Georgia, USA.ORCID https://orcid.org/0000-0001-7685-6460
Youssef M ZohdyDepartment of Neurosurgery, Emory University, Atlanta, Georgia, USA.
Karen Salmeron-MorenoDepartment of Neurosurgery, Emory University, Atlanta, Georgia, USA.ORCID https://orcid.org/0000-0003-0555-0063
Aliaksandr AksionauDepartment of Pathology, Emory University, Atlanta, Georgia, USA.ORCID https://orcid.org/0000-0003-0741-2000
Masayuki HiranoDepartment of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA.ORCID https://orcid.org/0000-0002-2455-9189
Samadhi SenanayakeDepartment of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA.
Razan FarajDepartment of Neurosurgery, Emory University, Atlanta, Georgia, USA.
Bethany ChernDepartment of Cell Biology, Emory University, Atlanta, Georgia, USA.
Hithardhi DuggireddyDepartment of Neurosurgery, Emory University, Atlanta, Georgia, USA.
Saikarthik PapisettyDepartment of Neurosurgery, Emory University, Atlanta, Georgia, USA.
Gustavo PradillaDepartment of Neurosurgery, Emory University, Atlanta, Georgia, USA.
Tomas Garzon-MuvdiDepartment of Neurosurgery, Emory University, Atlanta, Georgia, USA.ORCID https://orcid.org/0000-0002-2198-9757

Funding

Characterization of the Lamprey Adaptive Immune SystemR01AI072435 · NIAID · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI Max Dale Cooper · 2007 to 2026
$8.1M
T Cell Differentiation and Diversification in Jawless Vertebrates: Administrative Supplement for purchase of Laser Capture MicroscopeR35GM122591 · NIGMS · EMORY UNIVERSITY · PI Max Dale Cooper · 2017 to 2026
$4.7M
NIAID NIH HHS R01 AI072435NIGMS NIH HHS R35 GM122591
6 · The paper itself

Abstract

BACKGROUND AND

objectivesPreserving the quality and integrity of resected tumor tissue is essential to ensure the reliability of downstream analyses. The effects of ultrasonic aspirators (UAs) on cellular viability, tissue morphology, and molecular quality remain poorly characterized. This study directly compares tissue quality after collection by conventional sharp dissection (SD) vs UA.

methodsPaired tumor specimens were collected intraoperatively from 28 patients undergoing surgical resection of intracranial tumors. Samples collected by SD and UA were obtained from immediately adjacent regions. Cellular viability was assessed by automated cell counting. Tissue architecture was evaluated on hematoxylin and eosin-stained sections. Adherent monolayer cultures were established from paired specimens. RNA concentration and functional integrity were assessed by spectrophotometry and quantitative polymerase chain reaction of 4 housekeeping genes (GAPDH, TBP, HPRT1, and PGK1).

resultsCellular viability was higher in SD compared with UA specimens (80.25% ± 9.70% vs 72.33% ± 9.52%;

conclusionSD preserves cellular viability, RNA concentration, and tissue architecture more effectively than ultrasonic aspiration. However, both methods yielded tissue suitable for histopathological diagnosis and in vitro culture. These findings support a dual-collection strategy.

Indexed as

Cellular viabilityGlioblastomaPatient-derived tissueTissue integrityUltrasonic aspirator

Identifiers

PMID42666760
PMCPMC13524158

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.