ArticleFrontiers in microbiology2026
Comparative analysis of gut and ovarian microbiota in
Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Investigating the hidden microbial world within insect hosts yields new insights into their biology, ecology, and adaptation. Methods: We characterized bacterial communities in two tissue types (gut and ovary) of adult females and in whole-body samples of nymphs, collected from two ecologically contrasting regions of Morocco: Marrakech (arid, semi-desert climate) and Al Hoceima (humid, Mediterranean climate). Using 16S ribosomal RNA (rRNA) gene sequencing, we evaluated differences in diversity, composition, and relative abundance across regions and developmental stages. Results: Sequencing generated approximately 2.84 million reads across all samples, of which 91.9% were retained after quality filtering and contaminant removal, and 91.2% were successfully classified to bacterial taxa. Our results revealed clear geographic structuring of the microbiota. In insects from Marrakech, the adult gut and ovary harboured comparatively lower bacterial diversity and were dominated by Discussion/conclusion: Together, these findings highlight the spatial variability of insect-microbe association in
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