Evidence map›Paper›PMID 42665831›Full record

ArticleBMC plant biology2026

Identification of salt and drought resistance function of maize transcription factor ZmEREB54.

Yuqian Gao, Junxia Wang, Dengyu Zheng, Jie Zhao, Yuanyi Wu, Zhongyi Wu, Jinjie Guo, Chun Zhang

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Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Yuqian Gao *State Key Laboratory of North China Crop Improvement and Regulation, College of Agronomy, Hebei Agricultural University, Baoding, 071000, China.
Junxia Wang *Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
Dengyu ZhengBeijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
Jie ZhaoBeijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
Yuanyi WuBeijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
Zhongyi WuBeijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
Jinjie GuoState Key Laboratory of North China Crop Improvement and Regulation, College of Agronomy, Hebei Agricultural University, Baoding, 071000, China. guojinjie@hebau.edu.cn.
Chun ZhangBeijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China. zhangchun@baafs.net.cn.ORCID https://orcid.org/0000-0002-0442-6012

Funding

S&T Program of Hebei 24466301Dthe National Natural Science Foundation of China 32372053
6 · The paper itself

Abstract

backgroundsAP2/ERF (APETALA2/ethylene-responsive factor) represents one of the largest transcription factor superfamilies in plants, playing crucial roles in regulating plant growth and development as well as responding to abiotic stresses. Investigating the functions of maize (Zea mays L.) AP2/ERF family genes will provide novel genetic resources for maize genetic improvement.

resultsIn this study, the AP2/ERF transcription factor superfamily member ZmEREB54 (GRMZM2G020054, Gene ID: 100,278,463) was cloned from maize and was systematically analyzed functionally. The full-length CDS of ZmEREB54 gene was 561 bp, encoding 186 amino acids with a typical AP2/ERF conserved domain. Its promoter region contained cis-acting elements associated with responses to various abiotic stresses and hormones. Maize expression pattern analysis revealed that ZmEREB54 was highly expressed in V12 roots, with significant expression changes under osmotic stress, drought, high salinity, and treatments with abscisic acid (ABA) and jasmonic acid (JA). Phenotypic analysis showed that transgenic Arabidopsis thaliana over-expressing ZmEREB54 exhibited significantly longer roots compared to wild-type plants under high salinity, drought, osmotic stress, and hormone treatments (JA, ABA). Stress-responsive marker genes RD29A and RD22 were upregulated in the transgenic A. thaliana lines. The significantly decreased malondialdehyde (MDA) accumulation and markedly increased peroxidase (POD) activity in transgenic A. thaliana further demonstrate the improvement of its stress tolerance. Yeast two-hybrid (Y2H) assays revealed an interaction between ZmEREB54 and ZmMADS24.6, suggesting potential cooperative regulation of ZmEREB54 and ZmMADS24.6 in maize root development and stress responses.

conclusionThis study establishes a solid foundation for further clarifying the biological functions and molecular mechanisms of ZmEREB54 in regulating maize root growth and development, as well as responding to drought and salt stresses.

Indexed as

Plant ProteinsSalt ToleranceTranscription FactorsZea maysArabidopsisDrought ResistanceDroughtsGene Expression Regulation, PlantPhylogenyPlants, Genetically ModifiedStress, PhysiologicalPlant ProteinsTranscription FactorsAP2/ERF transcription factorDrought stressMaizeProtein interactionSalt stress

Identifiers

PMID42665831
PMCPMC13525665

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