Evidence map›Paper›PMID 42665788›Full record

ArticleBMC plant biology2026

Dissection of quantitative trait loci governing root system architecture under aluminium toxicity stress conditions in Brassica carinata-derived Brassica juncea introgression lines.

Shrusti Goud, Omkar Maharudra Limbalkar, Kanaka K K, Annappa Naik H J, Vyankatesh Dhanraj Bagul, Prashant Vasisth, Naveen Singh, Kishor U Tribhuvan, Santosh Kumar, Avinash Pandey and 2 more

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Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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12 authors.

Shrusti GoudIndian Council of Agricultural Research (ICAR)- Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand, India.
Omkar Maharudra LimbalkarIndian Council of Agricultural Research (ICAR)- Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand, India. omkarlimbalkar@gmail.com.
Kanaka K KIndian Council of Agricultural Research (ICAR)- Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand, India.
Annappa Naik H JIndian Council of Agricultural Research (ICAR)- Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand, India.
Vyankatesh Dhanraj BagulIndian Council of Agricultural Research (ICAR)- Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand, India.
Prashant VasisthDivision of Genetics, ICAR- Indian Agricultural Research Institute, New Delhi, India.
Naveen SinghDivision of Genetics, ICAR- Indian Agricultural Research Institute, New Delhi, India.
Kishor U TribhuvanIndian Council of Agricultural Research (ICAR)- Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand, India.
Santosh KumarICAR-Indian Agricultural Research Institute, Hazaribagh, Jharkhand, India.
Avinash PandeyIndian Council of Agricultural Research (ICAR)- Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand, India.
Vijai Pal BhadanaIndian Council of Agricultural Research (ICAR)- Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand, India.
Sujay RakshitIndian Council of Agricultural Research (ICAR)- Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAluminium (Al) toxicity in acidic soils is a major constraint limiting rapeseed-mustard productivity, particularly affecting Brassica juncea cultivation in acid-prone regions. The narrow genetic base of B. juncea restricts genetic improvement for Al toxicity stress tolerance. To enhance genetic variability and introgress beneficial genomic regions, Brassica carinata-derived B. juncea introgression lines (ILs) were developed and evaluated to dissect the genetic basis of root system architecture and stress tolerance under Al toxicity conditions.

resultsSignificant genetic variability was observed among the ILs for fourteen morpho-physiological traits evaluated under Al toxicity stress. Genotyping-by-sequencing generated 7,434 high-quality SNP markers, which were used to construct a high-density linkage map spanning 3,234.9 cM with an average marker interval of 2.3 cM. QTL analysis identified 24 significant QTLs, including 17 associated with root and shoot morphological traits and 7 linked to stress tolerance indices. Notable QTLs included qRV.1B.1 for root volume and qNF.3B.3 for number of forks, explaining 26.3% and 26.0% of phenotypic variation, respectively. Three QTL hotspots were detected, including a prominent region on chromosome 2B harboring QTLs for multiple stress tolerance indices. Several putative candidate genes with known roles in stress response pathways were identified within these QTL regions, including COBRA-like involved in cell wall expansion, peroxidase and cytochrome P450 associated with oxidative detoxification, and GATA, SAUR-like, zinc-knuckle, and DNA damage-inducible proteins involved in stress signalling and regulation.

conclusionsThis study elucidates the genetic architecture of Aluminium toxicity tolerance and identifies key QTLs and putative candidate genes governing root system architecture and stress adaptation in Brassica juncea introgression lines. The results demonstrate that interspecific introgression from B. carinata generated useful variation for root traits, enabling the identification of genomic regions associated with aluminium toxicity tolerance through GBS-based QTL mapping. These findings provide valuable genomic resources and promising targets for marker-assisted breeding to develop Aluminium toxicity-tolerant cultivars suitable for acidic soil environments.

Indexed as

AluminumMustard PlantPlant RootsQuantitative Trait LociChromosome MappingGenetic IntrogressionStress, PhysiologicalAluminumAbiotic stress toleranceAcid stressAl toxicityEthiopian mustardGenotyping by sequencingIndian mustard

Identifiers

PMID42665788
PMCPMC13523352

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